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anti mouse cd44 apc fire 750  (Bioss)


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    Bioss anti mouse cd44 apc fire 750
    (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg <t>CD44</t> + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.
    Anti Mouse Cd44 Apc Fire 750, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd44+apc+fire+750/pmc08356251-220-30-65?v=Bioss
    Average 91 stars, based on 1 article reviews
    anti mouse cd44 apc fire 750 - by Bioz Stars, 2026-08
    91/100 stars

    Images

    1) Product Images from "Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells"

    Article Title: Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells

    Journal: Life Science Alliance

    doi: 10.26508/lsa.202101056

    (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg CD44 + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.
    Figure Legend Snippet: (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg CD44 + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.

    Techniques Used: Expressing, Isolation

    (A) Experimental outline to induce MAV. Mice received 5 × 10 5 activated Pmel CD8 T cells expressing a luciferase reporter gene (Luc + Pmel) 5 d prior to tumor resection. (B) Luminescence signal of Luc + Pmel cells over the course of 21 d post tumor excision in unaffected and MAV-affected mice. (C) Representative flow plots showing expression of CXCR6 and CD44 on Luc + Pmel CD8 T cells. Cells were isolated from experimental mice on days 7, 14, or 35 post-surgery and were identified by CD8 + Thy1.1 + CD44 + ; Naïve Pmel CD8 T cells were isolated from naïve mice and identified by CD8 + Thy1.1 + CD44 − . Graphs are compiled data comparing the percentage of CXCR6 expression on CD8 + Thy1.1 + CD44 + (days 7, 14, and 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (D) CXCR6 mean fluorescence intensity expression on CD8 + Thy1.1 + CD44 + (day 7, 14, 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (E) Colocalization of CD8 and CXCR6 staining in skin from MAV-affected patients. Stains identify CD8 (green) and CXCR6 (pink); dark red indicates colocalization of CD8 and CXCR6. Scale bar, 25 μm. (F) Percent of CD8 T cells in skin from MAV-affected patients that are positive for CXCR6. (C, D, F) Symbols represent individual mice (C, D), individual patients (F); horizontal lines indicate mean. (C, D) n = 2–4; representative of three independent experiments (C, D). Significance was determined by one-way ANOVA; ** P ≤ 0.010.
    Figure Legend Snippet: (A) Experimental outline to induce MAV. Mice received 5 × 10 5 activated Pmel CD8 T cells expressing a luciferase reporter gene (Luc + Pmel) 5 d prior to tumor resection. (B) Luminescence signal of Luc + Pmel cells over the course of 21 d post tumor excision in unaffected and MAV-affected mice. (C) Representative flow plots showing expression of CXCR6 and CD44 on Luc + Pmel CD8 T cells. Cells were isolated from experimental mice on days 7, 14, or 35 post-surgery and were identified by CD8 + Thy1.1 + CD44 + ; Naïve Pmel CD8 T cells were isolated from naïve mice and identified by CD8 + Thy1.1 + CD44 − . Graphs are compiled data comparing the percentage of CXCR6 expression on CD8 + Thy1.1 + CD44 + (days 7, 14, and 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (D) CXCR6 mean fluorescence intensity expression on CD8 + Thy1.1 + CD44 + (day 7, 14, 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (E) Colocalization of CD8 and CXCR6 staining in skin from MAV-affected patients. Stains identify CD8 (green) and CXCR6 (pink); dark red indicates colocalization of CD8 and CXCR6. Scale bar, 25 μm. (F) Percent of CD8 T cells in skin from MAV-affected patients that are positive for CXCR6. (C, D, F) Symbols represent individual mice (C, D), individual patients (F); horizontal lines indicate mean. (C, D) n = 2–4; representative of three independent experiments (C, D). Significance was determined by one-way ANOVA; ** P ≤ 0.010.

    Techniques Used: Expressing, Luciferase, Isolation, Fluorescence, Staining



    Similar Products

    anti mouse cd44 apc fire 750  (Bioss)
    91
    Bioss anti mouse cd44 apc fire 750
    (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg <t>CD44</t> + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.
    Anti Mouse Cd44 Apc Fire 750, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd44+apc+fire+750/pmc08356251-220-30-65?v=Bioss
    Average 91 stars, based on 1 article reviews
    anti mouse cd44 apc fire 750 - by Bioz Stars, 2026-08
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg CD44 + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.

    Journal: Life Science Alliance

    Article Title: Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells

    doi: 10.26508/lsa.202101056

    Figure Lengend Snippet: (A) Representative flow plots showing expression of CD103 and CD69 on CD8 T cells isolated from LN and skin from melanoma-associated vitiligo (MAV)–affected mice. Cells were isolated from experimental mice 35 post-surgery and were identified by CD8 + CD62L neg CD44 + . (B) Skin from MAV-affected mice, stains identify CD8β (red), CD103 (green), and KLRG1 (white). Scale bar, 50 μm. (C) Number of CD103 neg KLRG1 neg CD8 + , CD103 + KLRG1 neg CD8 + , and CD103 neg KLRG1 + CD8 + T cells per cluster in a 10 μm thick skin section. Symbols represent individual clusters; horizontal lines indicate mean; n = 9 clusters. (D) Heat map of skin-resident memory transcripts comparing bulk endogenous CD8 T cells to Ag-specific Pmel CD8 T cells and naïve CD8 T cells. Bulk endogenous and Ag-specific Pmel CD8 T cells were isolated from MAV-affected skin, naïve CD8 T cells were isolated from spleen and LN of naïve Pmel mice.

    Article Snippet: Antibodies from BioLegend: anti-mouse CD45-APC/Fire 750 (clone 30-F11; #147713), anti-mouse CD8α-PerCP/Cynine5.5, -PE/Cy7 (clone 53-6.7; #100733, #100721), anti-mouse Thy1.1-PerCP/Cynine5.5 (clone OX-7; #202515), anti-mouse CD103-Alexa Fluor 647, -FITC (clone 2E7; #121409, #121419), anti-mouse CD44-APC/Fire 750 (clone IM7; #103061), anti-mouse CD62L-Brilliant Violet 510 (clone MEL-14; 104441), anti-mouse CXCR6-Brilliant Violet 421 (clone SA051D1; #151109), anti-mouse CD11c Alex Fluor 647 (clone N418; #117314), anti-mouse CD11b-Alexa Fluor 488 (clone M1/70; #101219); Bioss: anti-mouse CXCL16 Alexa Fluor 488 (polyclonal; #bs-1441R-A488).

    Techniques: Expressing, Isolation

    (A) Experimental outline to induce MAV. Mice received 5 × 10 5 activated Pmel CD8 T cells expressing a luciferase reporter gene (Luc + Pmel) 5 d prior to tumor resection. (B) Luminescence signal of Luc + Pmel cells over the course of 21 d post tumor excision in unaffected and MAV-affected mice. (C) Representative flow plots showing expression of CXCR6 and CD44 on Luc + Pmel CD8 T cells. Cells were isolated from experimental mice on days 7, 14, or 35 post-surgery and were identified by CD8 + Thy1.1 + CD44 + ; Naïve Pmel CD8 T cells were isolated from naïve mice and identified by CD8 + Thy1.1 + CD44 − . Graphs are compiled data comparing the percentage of CXCR6 expression on CD8 + Thy1.1 + CD44 + (days 7, 14, and 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (D) CXCR6 mean fluorescence intensity expression on CD8 + Thy1.1 + CD44 + (day 7, 14, 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (E) Colocalization of CD8 and CXCR6 staining in skin from MAV-affected patients. Stains identify CD8 (green) and CXCR6 (pink); dark red indicates colocalization of CD8 and CXCR6. Scale bar, 25 μm. (F) Percent of CD8 T cells in skin from MAV-affected patients that are positive for CXCR6. (C, D, F) Symbols represent individual mice (C, D), individual patients (F); horizontal lines indicate mean. (C, D) n = 2–4; representative of three independent experiments (C, D). Significance was determined by one-way ANOVA; ** P ≤ 0.010.

    Journal: Life Science Alliance

    Article Title: Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells

    doi: 10.26508/lsa.202101056

    Figure Lengend Snippet: (A) Experimental outline to induce MAV. Mice received 5 × 10 5 activated Pmel CD8 T cells expressing a luciferase reporter gene (Luc + Pmel) 5 d prior to tumor resection. (B) Luminescence signal of Luc + Pmel cells over the course of 21 d post tumor excision in unaffected and MAV-affected mice. (C) Representative flow plots showing expression of CXCR6 and CD44 on Luc + Pmel CD8 T cells. Cells were isolated from experimental mice on days 7, 14, or 35 post-surgery and were identified by CD8 + Thy1.1 + CD44 + ; Naïve Pmel CD8 T cells were isolated from naïve mice and identified by CD8 + Thy1.1 + CD44 − . Graphs are compiled data comparing the percentage of CXCR6 expression on CD8 + Thy1.1 + CD44 + (days 7, 14, and 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (D) CXCR6 mean fluorescence intensity expression on CD8 + Thy1.1 + CD44 + (day 7, 14, 35) or CD8 + Thy1.1 + CD44 − (naïve) cells isolated from skin, skin-draining LN, or spleen. (E) Colocalization of CD8 and CXCR6 staining in skin from MAV-affected patients. Stains identify CD8 (green) and CXCR6 (pink); dark red indicates colocalization of CD8 and CXCR6. Scale bar, 25 μm. (F) Percent of CD8 T cells in skin from MAV-affected patients that are positive for CXCR6. (C, D, F) Symbols represent individual mice (C, D), individual patients (F); horizontal lines indicate mean. (C, D) n = 2–4; representative of three independent experiments (C, D). Significance was determined by one-way ANOVA; ** P ≤ 0.010.

    Article Snippet: Antibodies from BioLegend: anti-mouse CD45-APC/Fire 750 (clone 30-F11; #147713), anti-mouse CD8α-PerCP/Cynine5.5, -PE/Cy7 (clone 53-6.7; #100733, #100721), anti-mouse Thy1.1-PerCP/Cynine5.5 (clone OX-7; #202515), anti-mouse CD103-Alexa Fluor 647, -FITC (clone 2E7; #121409, #121419), anti-mouse CD44-APC/Fire 750 (clone IM7; #103061), anti-mouse CD62L-Brilliant Violet 510 (clone MEL-14; 104441), anti-mouse CXCR6-Brilliant Violet 421 (clone SA051D1; #151109), anti-mouse CD11c Alex Fluor 647 (clone N418; #117314), anti-mouse CD11b-Alexa Fluor 488 (clone M1/70; #101219); Bioss: anti-mouse CXCL16 Alexa Fluor 488 (polyclonal; #bs-1441R-A488).

    Techniques: Expressing, Luciferase, Isolation, Fluorescence, Staining